Freezing and resuscitation-Mingqian Microbiology_Multifunctional mouse

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Freezing and resuscitation

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Freezing and resuscitation

In order to save space and reduce the occupancy rate of cages, it is possible to avoid physical or genetic pollution of mice due to factors such as external environment, microorganisms and genetic drift. Long-term stability can be achieved by freezing the genetic material in liquid nitrogen.

Freezing methods include sperm freezing and embryo freezing. The freezing and recovery comparison of the two freezing operations is as follows:

Freezing method	Sperm Freezing and Resuscitation	Embryo Freezing and Resuscitation
Amount of rats	≥ 2 fertile male rats aged 12-24 weeks	Three 12-24 week old fertile male rats, 15-40 less than 4 month old female rats
quantity of genetic material	15 to 20 straw sperm	Frozen 200/400 etc. Embryos
Technical features	IVF after sperm resuscitation yields large numbers of same-day-old offspring	Recipient can be transplanted directly after embryo resuscitation without IVF
Scope of application	Prefer single gene or common pure background lines (example: B6J,B6N)	Preference to monogenic/polygenic homozygous or special background lines